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ba 2000  (Vector Laboratories)


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    Structured Review

    Vector Laboratories ba 2000
    Ba 2000, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 7571 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+igg/Biotinylated+Horse+Anti-Mouse+IgG+Antibody/pmc13641933-71-45-44
    Average 96 stars, based on 7571 article reviews
    ba 2000 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Generated:

    Article Title: KDM3A catalyses the oxidation of acetyl-lysine to hydroxyacetyl-lysine on histone H3K9.
    Article Snippet: Purities were determined using an Agilent Technologies 1220 Infinity LC machine with a Phenomenex bioZen 3-μm Peptide PS-C18 LC column (150 × 4.6 mm), with the following linear gradient employed: 0–3 min (2% (vol/vol) B), 3–16 (2–25% (vol/vol) B), 16–17 (25–98% (vol/vol) B), 17–19 (98%(vol/vol) B), 19–20 (98–2% (vol/vol) B), 20–21 (2% (vol/vol) B). .. The antibodies used were actin (Sigma, A1978), Flag (Sigma, F1804), Flag (Sigma, F7425), HA (sc-7392), HA (CST, 3724S), HIF1α (BD Biosciences, 610959), H3K9ac (Abcam, ab4441, GR3290365-1, GR3229436-1, GR3253211-1, H3K9acOH fraction: R63, P1, F1.3 & F1.4, R43, P1, and F1.4, generated in this study), H3K9me3 (Abcam, ab8898), H3K9me2 (Abcam, ab1220), H3K9me1 (EpiCypher, 13-0014), H3K9me0 (Active Motif, 61399), histone H4 (Abcam, ab177840), KDM3A (Proteintech, 12835-1-AP), KDM3B (CST, 2621S), vinculin (Sigma, V9131), anti-rabbit immunoglobulin-G (IgG) (Vector, PI-1000-1) and anti-mouse IgG (Vector, PI-2000-1). ..

    Chromatin Immunoprecipitation:

    Article Title: KDM3A catalyses the oxidation of acetyl-lysine to hydroxyacetyl-lysine on histone H3K9.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used actin (Sigma, A1978, Lot: 065M4837V), Flag (Sigma, F1804, Lot: SLBN5629V), Flag (Sigma, F7425, Lot: 085M4774V), HA (sc-7392, Lot: G1818), HA (CST, 3724S, Lot: 8), HIF-1α (BD Biosciences, 610959, Lot: 5296905), H3K9ac (Abcam, ab4441, GR3290365-1, GR3229436-1, GR3253211), H3K9acOH fraction: R63, P1, F1.3 & F1.4, R43, P1, and F1.4 in this study), H3K9me3 (Abcam, ab8898, Lot: GR3217826-1, GR3245584-1), H3K9me2 (Abcam, ab1220, Lot: GR325223-3, GR3228498-2, GR325223-4, GR3308902-5), H3K9me1 (EpiCypher, 13-0014, Lot: 14247001), H3K9me0 (Active Motif, 61399, Lot: 34612001), Histone H4 (Abcam, ab177840, Lot: GR3189348-8), KDM3A (Proteintech, 12835-1-AP, Lot: 00009716), KDM3B (CST, 2621S, Lot: 1), Vinculin (sigma, V9131, Lot: N/A), anti-Rabbit IgG (Vector, PI-1000-1) and anti-Mouse IgG (Vector, PI-2000-1). ..

    Flow Cytometry:

    Article Title: KDM3A catalyses the oxidation of acetyl-lysine to hydroxyacetyl-lysine on histone H3K9.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used actin (Sigma, A1978, Lot: 065M4837V), Flag (Sigma, F1804, Lot: SLBN5629V), Flag (Sigma, F7425, Lot: 085M4774V), HA (sc-7392, Lot: G1818), HA (CST, 3724S, Lot: 8), HIF-1α (BD Biosciences, 610959, Lot: 5296905), H3K9ac (Abcam, ab4441, GR3290365-1, GR3229436-1, GR3253211), H3K9acOH fraction: R63, P1, F1.3 & F1.4, R43, P1, and F1.4 in this study), H3K9me3 (Abcam, ab8898, Lot: GR3217826-1, GR3245584-1), H3K9me2 (Abcam, ab1220, Lot: GR325223-3, GR3228498-2, GR325223-4, GR3308902-5), H3K9me1 (EpiCypher, 13-0014, Lot: 14247001), H3K9me0 (Active Motif, 61399, Lot: 34612001), Histone H4 (Abcam, ab177840, Lot: GR3189348-8), KDM3A (Proteintech, 12835-1-AP, Lot: 00009716), KDM3B (CST, 2621S, Lot: 1), Vinculin (sigma, V9131, Lot: N/A), anti-Rabbit IgG (Vector, PI-1000-1) and anti-Mouse IgG (Vector, PI-2000-1). ..

    Magnetic Resonance Imaging:

    Article Title: KDM3A catalyses the oxidation of acetyl-lysine to hydroxyacetyl-lysine on histone H3K9.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used actin (Sigma, A1978, Lot: 065M4837V), Flag (Sigma, F1804, Lot: SLBN5629V), Flag (Sigma, F7425, Lot: 085M4774V), HA (sc-7392, Lot: G1818), HA (CST, 3724S, Lot: 8), HIF-1α (BD Biosciences, 610959, Lot: 5296905), H3K9ac (Abcam, ab4441, GR3290365-1, GR3229436-1, GR3253211), H3K9acOH fraction: R63, P1, F1.3 & F1.4, R43, P1, and F1.4 in this study), H3K9me3 (Abcam, ab8898, Lot: GR3217826-1, GR3245584-1), H3K9me2 (Abcam, ab1220, Lot: GR325223-3, GR3228498-2, GR325223-4, GR3308902-5), H3K9me1 (EpiCypher, 13-0014, Lot: 14247001), H3K9me0 (Active Motif, 61399, Lot: 34612001), Histone H4 (Abcam, ab177840, Lot: GR3189348-8), KDM3A (Proteintech, 12835-1-AP, Lot: 00009716), KDM3B (CST, 2621S, Lot: 1), Vinculin (sigma, V9131, Lot: N/A), anti-Rabbit IgG (Vector, PI-1000-1) and anti-Mouse IgG (Vector, PI-2000-1). ..

    Amplification:

    Article Title: NKG2A inhibition promotes NK cell-CD8 + T cell interactions to improve anticancer immunity in ovarian carcinoma.
    Article Snippet: .. Detection was performed using the ImmPRESS® Excel Amplified Polymer Staining Kit, Anti-Mouse IgG, Peroxidase (MP-7602-15, Vector). .. Enzymatic activity was visualized using the ImmPACT DAB EqV Substrate Kit (Vector), and sections were counterstained with hematoxylin (DAKO).

    Polymer:

    Article Title: NKG2A inhibition promotes NK cell-CD8 + T cell interactions to improve anticancer immunity in ovarian carcinoma.
    Article Snippet: .. Detection was performed using the ImmPRESS® Excel Amplified Polymer Staining Kit, Anti-Mouse IgG, Peroxidase (MP-7602-15, Vector). .. Enzymatic activity was visualized using the ImmPACT DAB EqV Substrate Kit (Vector), and sections were counterstained with hematoxylin (DAKO).

    Staining:

    Article Title: NKG2A inhibition promotes NK cell-CD8 + T cell interactions to improve anticancer immunity in ovarian carcinoma.
    Article Snippet: .. Detection was performed using the ImmPRESS® Excel Amplified Polymer Staining Kit, Anti-Mouse IgG, Peroxidase (MP-7602-15, Vector). .. Enzymatic activity was visualized using the ImmPACT DAB EqV Substrate Kit (Vector), and sections were counterstained with hematoxylin (DAKO).

    Article Title: Epstein-Barr virus orchestrates spatial reorganization and immunomodulation in the classic Hodgkin lymphoma tumor microenvironment
    Article Snippet: Tissue sections were then blocked with 2.5% Normal Horse Serum (Vector Laboratories MP-7500-50) for 1 h at RT, and stained overnight at 4°C with anti-CD30 antibody diluted in antibody diluent buffer (1× TBS-T, 5% donkey serum, 0.05% sodium azide). .. Tissue slides were then washed twice in washing buffer, and then stained with secondary anti-mouse IgG and anti-rabbit IgG HRP-conjugated antibodies for 30 min at RT (Vector Laboratories, MP-7500-50). .. After washing twice in washing buffer for 5 min each, the DAB peroxidase substrate (Vector Laboratories SK-4100) was introduced, and brown coloration was allowed to develop over 2.5 min. Tissue slides were briefly rinsed in tap water and counterstained with hematoxylin (Vector Laboratories H3404-100) for 2 min, and blue coloration was allowed to develop by rinsing in tap water for five times at 3 min each.

    Sterility:

    Article Title: Therapeutic targeting of blood-derived protein infiltration to modulate neuroinflammation in cerebellar ataxia.
    Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS Technologies, Inc., Cat#: HCP2‐0010; Essex Junction, VT, USA), 10 kDa fluorescein isothiocyanate (FITC)–dextran (Thermo Fisher Scientific, Cat#: D1820, Waltham, MA, USA), rivaroxaban (Selleckchem, Cat#: 366789‐02‐8; Houston, TX, USA), caffeine (Sigma-Aldrich, Cat#: 58‐08‐2; St. Louis, MO, USA), rabbit anti‐zonula occludens‐1 (ZO‐1; Thermo Fisher Scientific, Cat#: 61‐7300; Waltham, MA, USA), rabbit anti‐cluster of differentiation 31 (CD31; Thermo Fisher Scientific, Cat#: PA5‐16301, Waltham, MA, USA), sheep anti‐prothrombin fragment 2 (pKr-2 marker; Fitzgerald Industries, Cat#: 70R‐10587; Acton, MA, USA), mouse anti‐prothrombin (which recognizes an epitope in the pKr‐2 domain of prothrombin; Haematologic Technologies Inc., Cat#: AMP‐9013, VT, USA), rabbit anti‐prothrombin (Thermo Fisher Scientific, Cat#: PA5‐77976; Rockford, IL, USA), rabbit anti‐thrombin (Abcam, Cat#: ab92621; Cambridge, UK), rabbit anti-calbindin (Swant, Cat#: CB38, Burgdorf, Switzerland), rabbit anti-neuronal nuclei (NeuN; Cell Signaling Technology, Cat#: CST24306, Beverly, MA, USA), rabbit anti‐ionized calcium‐binding adapter molecule 1 (Iba1; Wako Pure Chemical Industries, Cat#: 019‐19741; Osaka, Japan), rabbit anti-glial fibrillary acidic protein (GFAP; Abcam, Cat#: ab68428, Cambridge, UK), rabbit anti-tumor necrosis factor-alpha (TNF-α, Abcam, Cat#: ab6671, Cambridge, UK), rabbit anti-interleukin-1 beta (IL-1β, Abcam, Cat#: ab9722, Cambridge, UK), rabbit anti-inducible nitric oxide synthase (iNOS, Abcam, Cat#: ab3523; Cambridge, UK), rat anti-cluster of differentiation 86 (CD86; Thermo Fisher Scientific, Cat# : 14-0862-81, Waltham, MA, USA), rabbit anti-toll like receptor 4 (TLR4; Santa Cruz Biotechnology, Cat#: sc‐293072, .. AR TIC LE IN PR ES S ARTICLE IN PRESS Dallas, TX, USA), rabbit anti-phospho-c-Jun (p-c-Jun; Cell Signaling Technology, Cat#: CST3270, Beverly, MA, USA), rabbit anti-c-Jun (Cell Signaling Technology, Cat#: CST9165S, Beverly, MA, USA), rabbit antiPU.1 (Cell Signaling Technology, Cat#: CST2258, Beverly, MA, USA), rabbit anti-ataxin-2 (ProteinTech, Cat#: 21776-1-AP; Rosemont, IL, USA), and mouse anti‐β‐actin (Santa Cruz Biotechnology, Cat#: sc‐47778; Dallas, TX, USA). biotinylated anti‐rabbit immunoglobulin G (IgG; Vector Laboratories, Cat#: BA‐1000, Burlingame; CA, USA), Texas Redconjugated anti-rabbit IgG (Vector Laboratories, Cat#: TI-1000, Burlingame, CA, USA), FITC-conjugated anti-mouse IgG (Vector Laboratories, Cat#: FI-2000, Burlingame, CA, USA), Alexa Fluor 488 antirat IgG (Thermo Fisher Scientific, Cat#: A-21208, Waltham, MA, USA), horseradish peroxidase (HRP)‐conjugated anti‐rabbit IgG (Enzo Life Sciences, Cat#: ADI‐SAB‐300; Farmingdale, NY, USA), HRP‐conjugated anti‐mouse IgG (Thermo Fisher Scientific, Cat#: 61‐6520; Waltham, MA, USA), HRP‐conjugated anti‐sheep IgG (GenWay Biotech Inc., Cat#: GWB‐3E18C4; San Diego, CA, USA), sterile phosphate-buffered saline (PBS) was used as the vehicle to dissolve pKr‐2. ..

    Saline:

    Article Title: Therapeutic targeting of blood-derived protein infiltration to modulate neuroinflammation in cerebellar ataxia.
    Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS Technologies, Inc., Cat#: HCP2‐0010; Essex Junction, VT, USA), 10 kDa fluorescein isothiocyanate (FITC)–dextran (Thermo Fisher Scientific, Cat#: D1820, Waltham, MA, USA), rivaroxaban (Selleckchem, Cat#: 366789‐02‐8; Houston, TX, USA), caffeine (Sigma-Aldrich, Cat#: 58‐08‐2; St. Louis, MO, USA), rabbit anti‐zonula occludens‐1 (ZO‐1; Thermo Fisher Scientific, Cat#: 61‐7300; Waltham, MA, USA), rabbit anti‐cluster of differentiation 31 (CD31; Thermo Fisher Scientific, Cat#: PA5‐16301, Waltham, MA, USA), sheep anti‐prothrombin fragment 2 (pKr-2 marker; Fitzgerald Industries, Cat#: 70R‐10587; Acton, MA, USA), mouse anti‐prothrombin (which recognizes an epitope in the pKr‐2 domain of prothrombin; Haematologic Technologies Inc., Cat#: AMP‐9013, VT, USA), rabbit anti‐prothrombin (Thermo Fisher Scientific, Cat#: PA5‐77976; Rockford, IL, USA), rabbit anti‐thrombin (Abcam, Cat#: ab92621; Cambridge, UK), rabbit anti-calbindin (Swant, Cat#: CB38, Burgdorf, Switzerland), rabbit anti-neuronal nuclei (NeuN; Cell Signaling Technology, Cat#: CST24306, Beverly, MA, USA), rabbit anti‐ionized calcium‐binding adapter molecule 1 (Iba1; Wako Pure Chemical Industries, Cat#: 019‐19741; Osaka, Japan), rabbit anti-glial fibrillary acidic protein (GFAP; Abcam, Cat#: ab68428, Cambridge, UK), rabbit anti-tumor necrosis factor-alpha (TNF-α, Abcam, Cat#: ab6671, Cambridge, UK), rabbit anti-interleukin-1 beta (IL-1β, Abcam, Cat#: ab9722, Cambridge, UK), rabbit anti-inducible nitric oxide synthase (iNOS, Abcam, Cat#: ab3523; Cambridge, UK), rat anti-cluster of differentiation 86 (CD86; Thermo Fisher Scientific, Cat# : 14-0862-81, Waltham, MA, USA), rabbit anti-toll like receptor 4 (TLR4; Santa Cruz Biotechnology, Cat#: sc‐293072, .. AR TIC LE IN PR ES S ARTICLE IN PRESS Dallas, TX, USA), rabbit anti-phospho-c-Jun (p-c-Jun; Cell Signaling Technology, Cat#: CST3270, Beverly, MA, USA), rabbit anti-c-Jun (Cell Signaling Technology, Cat#: CST9165S, Beverly, MA, USA), rabbit antiPU.1 (Cell Signaling Technology, Cat#: CST2258, Beverly, MA, USA), rabbit anti-ataxin-2 (ProteinTech, Cat#: 21776-1-AP; Rosemont, IL, USA), and mouse anti‐β‐actin (Santa Cruz Biotechnology, Cat#: sc‐47778; Dallas, TX, USA). biotinylated anti‐rabbit immunoglobulin G (IgG; Vector Laboratories, Cat#: BA‐1000, Burlingame; CA, USA), Texas Redconjugated anti-rabbit IgG (Vector Laboratories, Cat#: TI-1000, Burlingame, CA, USA), FITC-conjugated anti-mouse IgG (Vector Laboratories, Cat#: FI-2000, Burlingame, CA, USA), Alexa Fluor 488 antirat IgG (Thermo Fisher Scientific, Cat#: A-21208, Waltham, MA, USA), horseradish peroxidase (HRP)‐conjugated anti‐rabbit IgG (Enzo Life Sciences, Cat#: ADI‐SAB‐300; Farmingdale, NY, USA), HRP‐conjugated anti‐mouse IgG (Thermo Fisher Scientific, Cat#: 61‐6520; Waltham, MA, USA), HRP‐conjugated anti‐sheep IgG (GenWay Biotech Inc., Cat#: GWB‐3E18C4; San Diego, CA, USA), sterile phosphate-buffered saline (PBS) was used as the vehicle to dissolve pKr‐2. ..

    Incubation:

    Article Title: BTG1 Acts as a Critical Tumor Suppressor Link Between HDAC Inhibition and β-Catenin Signaling Suppression in Diffuse Large B-Cell Lymphoma.
    Article Snippet: Diffuse large B‐cell lymphoma (DLBCL) is an aggressive hematologic malignancy with complex pathogenesis and unsatisfactory clinical outcomes.. Although histone deacetylase inhibitors (HDACis) have demonstrated therapeutic potential, the molecular mechanisms linking epigenetic regulation to key oncogenic signaling pathways remain incompletely understood.. In this study, B‐cell translocation gene 1 (BTG1) was identified as a critical tumor suppressor that is epigenetically upregulated by HDAC inhibition in DLBCL cells.



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    Image Search Results


    Journal: iScience

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12

    doi: 10.1016/j.isci.2026.117719

    Figure Lengend Snippet:

    Article Snippet: Anti-mouse IgG (H + L)-HRP , Bio-Rad , RRID: AB_2617113.

    Techniques: Cell-Signaling, Virus, Recombinant, Clinical Proteomics, Membrane, Staining, Cloning, Software

    Journal: iScience

    Article Title: Midline thalamic neurons show early hypersynchrony during absence seizures in mouse models

    doi: 10.1016/j.isci.2026.117673

    Figure Lengend Snippet:

    Article Snippet: Sections were then washed in 1 x PBS four times and incubated in Alexa Fluor 488 AffiniPure Donkey Anti-Mouse IgG (H + L, Jackson ImmunoResearch, cat no: 715–545-150, West Grove, Pennsylvania), 1:200 dilution in 1% normal donkey serum in 0.5% Triton X-100 overnight at 4°C.

    Techniques: Recombinant, Mutagenesis, Software, Analysis, Silicon